Fig. 5: Inhibition of the SE-associated key targets attenuated LUAD malignant progression via the suppression of master TFs in CRC model.

A–C Perturbation of SE associated key targets, including BRD4, EP300, and CDK7, by small-molecule inhibitors reduced the expression of master TFs significantly at protein levels. D Expressions of master TFs in LUAD cell line PC-9 treated with SE associated targets inhibitors in mRNA level were analyzed by real-time PCR. E Inhibition of invasive and migrate activity by perturbation of SE associated key targets via small molecular inhibitions. PC-9 cells were cultured with indicated small molecular inhibitions and subjected to invasion and migration assays (see “Materials and methods”). Invaded and migrated cells were stained with crystal violet and counted. Representative photographs were shown. F, G Histograms represent the number of invasion or migration cells. H In PC-9 cell lines, alterations of ELF3, EHF, and TGIF1 at protein levels while JQ1 treated for 100 nM 48 h together with or without transfected with overexpression plasmids. GAPDH and β-actin were used as internal control, the lower panels showed the gray scale ratio of protein (ELF3) to GAPDH and protein (EHF and TGIF1) to β-actin. *p < 0.05; **p < 0.01; ***p < 0.001.