Fig. 4: Upregulation of GLT8D2 activates the FGFR/PI3K signalling axis in ovarian cancer. | Oncogenesis

Fig. 4: Upregulation of GLT8D2 activates the FGFR/PI3K signalling axis in ovarian cancer.

From: Overexpression of glycosyltransferase 8 ___domain containing 2 confers ovarian cancer to CDDP resistance by activating FGFR/PI3K signalling axis

Fig. 4

a Lysates from Flag- GLT8D2 overexpression cancer cells were immunoprecipitated using anti-Flag affinity agarose, followed by mass-spectrometric peptide sequencing. b Co-IP assay showing that GLT8D2 interacted with FGFR1. c Western blotting analysis of the phosphorylation levels of FRS2a and AKT in the indicated cells. GADPH was used as a loading control. d GSEA analysis showing that GLT8D2 expression was correlated with PI3K/AKT target gene signatures in TCGA ovarian cancer datasets. e Analysis of luciferase reporter activity in the indicated cells after transfection with 100 ng FOXO-luciferase plasmids or control-luciferase plasmid, * P < 0.05. f Western blotting analysis of the expression levels the phosphorylation levels of AKT and GSK3b in the indicated cells. GADPH was used as a loading control.

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