Fig. 1: Upregulated CXCR2 is associated with increased TCF7L1 of PCa.

Volcano plot of differentially expressed genes between CXCR2⁺ NE and CXCR2⁻ luminal cells analyzed from transcriptome profile data downloaded from GEO (GSE114326). TCF7L1 is a gene which is significantly upregulated in the population of CXCR2⁺ NE-enriched cells. B GSEA showing that higher TCF7L1 expression was positively associated with the CXCR2⁺ NE-responsive signature [36] and NE-responsive signature [38] in TCGA PCa dataset. NES normalized enrichment score, FDR false discovery rate. C GSEAs of TCGA PCa dataset showing that higher TCF7L1 expression was positively associated with neuronal development (GO and KEGG) and negatively associated with androgen-responsive signaling (GO, PID, Wang [39], and Nelson [40] gene signatures). D, E Images and intensity of consecutive tissue sections stained with TCF7L1 and CXCR2 in SCPC samples collected from Taipei Medical University-Wan Fang Hospital. n = 11. Scale bars, 100 μm. The significance of the positive correlation among the intensities of TCF7L1 and CXCR2 was determined by two-tailed Pearson correlation XY analyses in GraphPad Prism. R, correlation coefficient, P, p (two-tailed) value of SCPC samples. F, G Images and intensity of consecutive tissue sections stained for TCF7L1 and CXCR2 in a CRPC TMA from the Duke University School of Medicine. Scale bars, 100 μm. Higher TCF7L1 expression was positively associated with higher CXCR2-expressing PCa samples as observed in the CRPC TMA (n = 25). The significance of the positive correlation among the intensities of TCF7L1 and CXCR2 was determined by two-tailed Pearson correlation XY analyses in GraphPad Prism. R, correlation coefficient, P, p (two-tailed) value. H GSEA of TCGA PCa dataset showing that enrichment of TCF7L1 expression was positively associated with an upregulated IL-8/CXCR2 responsive signature (PID).