Fig. 1: SIK1 expression is regulated by EWSR1-WT1.
From: Salt-Inducible Kinase 1 is a potential therapeutic target in Desmoplastic Small Round Cell Tumor
A Heatmap of relative transcript levels of SIK-family and other kinases in DSRCT, ARMS, ASPS, ES, SS primary tumors based on Affymetrix U133A expression array data. B EWSR1-WT1 and SIK1 protein levels in dox-inducible shRenilla or shWT1 JN and BER-DSRCT stable cell lines. C Relative mRNA expressions of EWSR1-WT1, SIK1, SIK2, and SIK3 in JN and BER-DSRCT shRenilla or shWT1 inducible cell lines with or without Dox treatment. D Relative mRNA expression of SIK1, SIK2, and SIK3 in TET-off inducible UF5 (E - KTS) and UED5 (E+KTS) cells (*p < 0.05, **p < 0.01, ***p < 0.001, mean ± SEM, student t-test). E Relative mRNA expressions of EWSR1-WT1, SIK1, SIK2, and SIK3 in LP9 cells that have been transduced with a control lentivirus (empty vector (EV)) or with lentiviruses expressing E-KTS, E+KTS, or both isoforms (E−/+KTS). mRNA expression in transduced cells were compared to the non-transduced LP9 cells. (**p < 0.01, ***p < 0.001, ****p < 0.0001, mean ± SEM, student t-test).
