Fig. 4: SIK1 is essential for DNA replication in DSRCT cells.
From: Salt-Inducible Kinase 1 is a potential therapeutic target in Desmoplastic Small Round Cell Tumor
A Heatmap of commonly regulated genes following EWSR1-WT1 or SIK1 depletion in DSRCT cells. B Top common pathways that were altered in IPA (Qiagen) following EWSR1-WT1 or SIK1 depletion in DSRCT cells. C Cell cycle analysis of G1-arrested shRenilla or shSIK1 DSRCT cell lines with or without Dox treatment following release to S phase. Quantification from three independent experiments are shown. D EdU incorporation analysis of dox-inducible shRenilla and shSIK1 BER-DSRCT stable cell lines. A representative flow cytometry data from three independent experiments are shown in the upper panel. Percent EdU+ cells (lower panel) were calculated from three independent experiments (***p < 0.001, ****p < 0.0001, mean ± SEM, student t-test). E SIK1 depletion reduces MCM2 phosphorylation at S27 and S41 sites. Western blot with specific phospho-specific antibodies to MCM2 S27, S41, S139, and MCM2. Phosphorylation signal intensity was normalized to total MCM2 levels (n = 3, *p < 0.05, ***p < 0.001 mean ± SEM, student t-test).
