Fig. 1: The cyclin D1 carboxyl terminal E-rich region is necessary for the recognition of modified histones by Surface plasmon resonance. | Oncogenesis

Fig. 1: The cyclin D1 carboxyl terminal E-rich region is necessary for the recognition of modified histones by Surface plasmon resonance.

From: A cyclin D1 intrinsically disordered ___domain accesses modified histone motifs to govern gene transcription

Fig. 1

A Coomassie staining and (B). Western blot for cyclin D1 (CD1WT), cyclin D1 E-region deleted (CD1ΔE) and KDM4A GST fusion proteins. C GST pull-down, with (D). quantitation shown as mean ± SEM for multiplicate experiments for GST-CD1WT, GST-CD1ΔE or GST-ctrl fusion proteins with histone peptide (H2B) and S14-phosphorylated histone peptide (H2BS14P). In comparison, GST pull-down of histone H3K4me3 with KDM4A is shown in (E). F Schematic representation of GST-cyclin D1 wild type and ΔE mutant. G Surface plasmon resonance (SPR) analysis of histone peptide (H2BS14P) with GST-cyclin D1, GST-ΔE or GST-ctrl fusion proteins. (GST-Cyclin D1: blue:10 μM, light blue:7 μM, magenta:5 μM, red:3.5 μM, orange:1 μM).

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