Fig. 3: Knockdown of SMYD3 impairs tumorigenicity and metastasis of EC in vivo. | Oncogenesis

Fig. 3: Knockdown of SMYD3 impairs tumorigenicity and metastasis of EC in vivo.

From: SMYD3 promotes endometrial cancer through epigenetic regulation of LIG4/XRCC4/XLF complex in non-homologous end joining repair

Fig. 3

A Western blotting of the indicated proteins in WCLs from HEC1B cells stably expressing shRFP, shSMYD3-1, or shSMYD3-2. B Images of HEC1B cells stably expressing shRFP, shSMYD3-1, or shSMYD3-2-implanted tumors from nude mice. C The weight of xenograft tumors shown in B. n = 7. D The tumor volume of xenograft tumors was measured on the indicated days after injection. E Representative images of immunofluorescence staining of SMYD3, Ki67, and cleaved-Caspase3. Scale bar, 30 μm. F Schematic diagram of cell inoculation and liver metastases in nude mice. G Images of HEC1B cells stably expressing shRFP, shSMYD3-1, or shSMYD3-2 liver metastases from nude mice (Upper graph). HE staining of HEC1B cells stably expressing shRFP, shSMYD3-1, or shSMYD3-2 liver metastases from nude mice (Lower graph). Scale bar, 30 μm.

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