Fig. 4: SMYD3 is recruited to DNA damage sites in a PARP1-dependent manner.
A The top upregulated Gene Ontology (GO) terms in the enrichment analysis. B Western blotting of the indicated proteins in WCLs from HEC1B cells after γ-ray irradiation at 10 Gy (Left graph). Western blotting of the indicated proteins in WCLs treated with 40 μM VP16 for 1, 2, and 4 h (Right graph). C Western blotting of chromatin proteins extracted from HEC1B cells after γ-ray irradiation at 10 Gy (Left graph). Western blotting of chromatin proteins extracted from HEC1B cells treated with 40 μM VP16 for 1, 2, and 4 h (Right graph). D Western blotting of the indicated proteins in WCLs from U2OS cells transfected with indicated plasmids. E Dynamics of GFP-SMYD3 or GFP-SMYD3F183A accumulation at micro-irradiated sites (Left graph). Relative fluorescence intensity of GFP-SMYD3 and GFP-SMYD3F183A at micro-irradiated sites in the experiments described above (Right graph). Scale bar, 5 μm. F Dynamics of GFP-SMYD3 accumulation at micro-irradiated sites treated with different inhibitors at the indicated concentration for 24 h (Left graph). Relative fluorescence intensity of GFP-SMYD3 at micro-irradiated sites in the experiments described in the left graph (Right graph). Scale bar, 5 μm. G Western blotting of the indicated proteins in WCLs from U2OS cells stably expressing shRFP, shPARP1. H Time-lapse images of GFP-SMYD3 accumulation at micro-irradiated sites in U2OS cells stably expressing shRFP or shPARP1 (Left graph). Relative fluorescence intensity of GFP-SMYD3 at micro-irradiated sites in the experiments described in the left graph (Right graph). Scale bar, 5 μm.
