Fig. 3: The interaction of EPHA5 with STAT3 and the expressions of STAT3-mediated transcription downstream targets. | Oncogenesis

Fig. 3: The interaction of EPHA5 with STAT3 and the expressions of STAT3-mediated transcription downstream targets.

From: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway

Fig. 3

A Interaction network with EPHA5, from Biogrid database. B Co-immunoprecipitation experiments were conducted on FTC-238 cells lysates with anti-EPHA5 and anti-STAT3, and then the precipitation was subsequently analyzed by western blotting with anti-STAT3 and anti-EPHA5. C Immunofluorescence analyses were performed using anti-EPHA5 antibodies or anti-STAT3 antibodies. Representative images are shown. Scale bar, 25 μm. D Western blot analysis of STAT3, p-STAT3, β-actin in FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 respectively. **p < 0.01. n = 3. E QRT-PCR analysis of BCL-XL, Myc, Timp-1, and CyclinD1 mRNA in FTC-133 and FTC-238 cells with EPHA5 knockdown. *p < 0.05; **p < 0.01, n = 8.

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