Fig. 4: P-STAT3 inhibition inhibited proliferation and enhanced apoptosis of FTC cells with EPHA5 knockdown. | Oncogenesis

Fig. 4: P-STAT3 inhibition inhibited proliferation and enhanced apoptosis of FTC cells with EPHA5 knockdown.

From: EPHA5 promotes cell proliferation and inhibits apoptosis in Follicular Thyroid Cancer via the STAT3 signaling pathway

Fig. 4

A Western blot analysis of PCNA, p-STAT3, STAT3, Caspase-3, Cleaved-caspase3 and β-actin in FTC-133 and FTC-238 cells. *p < 0.05; **p < 0.01, n = 3. B Colony formation assay of FTC-133 and FTC-238 cell. **p < 0.01, n = 6. C Representative TUNEL staining of FTC cells; the nuclei were stained with DAPI. Scale bar = 200 μm. TUNEL scores. The relative number of apoptotic cells is represented as TUNEL-positive cells/DAPI. **p < 0.01, n = 6. D Western blot analysis of STAT3, cleaved-caspase-3, and β-actin in FTC-133 and FTC-238 cells. **p < 0.01, n = 3. E Colony formation assay of FTC-133 and FTC-238 cells transfected with si-NC or si-EPHA5 or si-EPHA5 + si-STAT3 respectively. *p < 0.05, **p < 0.01, n = 3. F Representative FITC and PI staining of FTC cells. Scale bar, 200 μm. The relative number of apoptotic cells is represented as FITC-positive cells ratio. **p < 0.01, n = 3.

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