Fig. 5: EGF promoted IL-8 expression in L-02 cells by activating the ERK1/2 pathway, and EGF activated LX-2 cells.
From: Potential therapeutic target of EGF on bile duct ligation model and biliary atresia children
a Expression of IL-8 mRNA was elevated in L-02 cells after EGF intervention. b mRNA expression of IL-8 increased with the increase of EGF concentration. c KO-947 inhibited the overexpression of IL-8 induced by EGF. ns: P > 0.05; ***P < 0.001; ****P < 0.0001. After treating LX-2 cells with EGF for 24 h. d–g qRT-PCR showed mRNA expression of ACTA2, COLI1A1, COLI1A2 and S100A4 was upregulated. **P < 0.01; ***P < 0.001. h EGF promoted α-SMA expression in LX-2 cells in a concentration-dependent manner. i IF staining displayed that EGF promoted α-SMA expression in LX-2 cells. (Scale bar: 100 μm).
