Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: Chemical compound cinobufotalin potently induces FOXO1-stimulated cisplatin sensitivity by antagonizing its binding partner MYH9

Fig. 4

MYH9 interacts with GSK3β and degrades GSK3β by ubiquitination in FOXO1-overexpressing NPC cells. a Co-IP detected the interaction of exogenous GSK3β and MYH9 in 5–8F cells. b Cytosolic colocalization of GSK3β protein and MYH9 protein in NPC cells was visualized by immunofluorescence staining. Scale bar, 25 μm. c GSK3β expression was measured in MYH9-overexpressing NPC cells; one-way ANOVA and Dunnett’s multiple comparison test were used for analysis. d, e Western blotting and quantification analysis were performed to analyze the effect of MYH9 overexpression on GSK3β and β-catenin stability in NPC cells treated with cycloheximide at different time points and with the presence of MG132. f Coimmunoprecipitation analysis of the effect of FOXO1 and MYH9 on the interaction between GSK3β, MYH9, ubiquitin, and TRAF6 in FOXO1-overexpressing NPC cells. g Coimmunoprecipitation analysis of GSK3β, TRAF6, and ubiquitin in NPC cells treated with wild-type GSK3β or mutant GSK3β. h Nuclear and cytoplasmic extraction assays showed protein levels of β-catenin in the nucleus and cytoplasm of FOXO1-overexpressing NPC cells

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