Fig. 3
From: Inactivated SARS-CoV-2 induces acute respiratory distress syndrome in human ACE2-transgenic mice
IMMs contribute to the progress of ARDS induced by inactivated SARS-CoV-2. a Flow cytometric analysis of neutrophils (labeled as Ly6G+), inflammatory monocyte-macrophages (IMMs, labeled as CD11b+ Ly6Chi) accumulations in lung tissues and correlated levels of activation markers (CD69 and CD80) on 3 dpi. The treated hACE2 mice showed high levels of pulmonary infiltration of activated neutrophils and IMMs. b–e Elimination of IMMs by i.v. injection of clophosome-A showed decreased lung weight and ameliorative pulmonary pathology, including lower interstitial edema, inflammatory cell infiltration, and pulmonary injury, in treated hACE2 mice. Images of lung tissues (b), measurement of lung weight (c), images of H&E staining (d) and pulmonary pathological scores for mice according to the scoring system (e) on 3 dpi. f–i Elimination of pulmonary resident macrophages by p.a. administration of clophosome-A showed an exacerbated pulmonary pathology in treated hACE2 mice. Images of lung tissues (f), lung weight (g), images of H&E staining (h) and pulmonary pathological scores for mice according to the scoring system (i) on 3 dpi. i.v., intravenous; p.a., pharyngeal aspiration. Data represent the mean ± SEM. Significance is indicated by: ns, no significance; *P ≤ 0.05, **P ≤ 0.01
