Fig. 8
From: Methylation across the central dogma in health and diseases: new therapeutic strategies
Principle of site-specific manipulating DNA, histone, and RNA methylation. a Three generations of programmable DNA-binding ___domain (DBD) platforms for site-specific chromatin methylation manipulation: zinc fingers (ZFs), transcription activator-like effectors (TALEs), and catalytically dead CRISPR-dCas system. b Fusion of dCas9 to DNA methyltransferases (e.g., DNMT3A) or demethylases (e.g., TET1) allows for targeted DNA methylation or demethylation, respectively. c Fusion of dCas9 to protein methylation machinery (e.g., KRAB) or demethylases (e.g., LSD1) enables artificial histone methylation or demethylation at defined loci, respectively. d Fusion of dCas13 to RNA methyltransferases (e.g., METTL3) or demethylases (e.g., FTO) allows for selective deposition or removal of m6A signals at specific transcripts, respectively
