Fig. 3 | Signal Transduction and Targeted Therapy

Fig. 3

From: Single-cell exome sequencing reveals polyclonal seeding and TRPS1 mutations in colon cancer metastasis

Fig. 3

Subclonal structure within 10 metastatic colon cancers and single-cell RNA-seq analysis of primary CRC and matched liver metastases samples of 6 CRLM patients. a Monoclonal seeding of 4 patients is shown as phylogenetic trees. b Polyclonal seeding of 4 patients is shown as phylogenetic trees. C: colon cancer (primary tumor), L: liver metastases, C + L: colon cancer and liver metastases. The relative branch length of the phylogenetic tree reflects the percentage of all mutations in a cluster. c UMAP plot segregating cancer cells of primary CRC into 8 subgroups. Colors indicate different subgroups. d–f UMAP plot showing the MC score, EMT score, CNV score of each single cell. Cells are colored according to MC score. g Sankey plots showing the associations between subgroups of cancer cells in primary CRC and matched liver metastases. h Histogram showing the proportion of tumor cells in primary CRC that metastasized to the liver in each CRLM patient. i Scatter plot with trend line showing a significant positive linear correlation between the EMT score and MC score

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