Fig. 2: Bacteria produce and secrete NO under oxic conditions.
From: Aerobic bacteria produce nitric oxide via denitrification and promote algal population collapse
Relative gene expression of P. inhibens denitrification genes under exposure to 10 µM nitrite for the indicated times; (A) nirK, (B) norB. Each data point consists of 3 biological replicates, error bars designate ±SD. C Microscopy images of WT and Δ262 bacteria cured from the native 262 kb plasmid, stained with the fluorescent NO-indicator diacetate DAF-FM, and incubated with or without 100 µM nitrite for 2 h. Scale bar corresponds to 1 µm. D Extracellular nitric oxide concentrations of WT and Δ262 bacteria incubated with the indicated nitrite concentrations. Each data point consists of 3 biological replicates, each containing 107 cells/ml. Error bars designate ±SD. ND stands for not detected.
