Fig. 1: Chemogenetic activation of PV-IN enhances the GABAergic tone onto CA1 pyramidal neurons. | Translational Psychiatry

Fig. 1: Chemogenetic activation of PV-IN enhances the GABAergic tone onto CA1 pyramidal neurons.

From: Chemogenetic rectification of the inhibitory tone onto hippocampal neurons reverts autistic-like traits and normalizes local expression of estrogen receptors in the Ambra1+/- mouse model of female autism

Fig. 1

A Breeding. B Experimental design: AAV-hSyn-DIO-hM3Dq-mCherry (hM3Dq) was bilaterally injected in CA1. Tests were performed 4 weeks later. Vehicle (Veh) or CNO were injected i.p. 40 min before behavioral testing and electrophysiological recordings, and 70 min before rtPCR experiments, or diluted at the same concentration in the drinking water 24 h before mice were sacrificed for dendritic spine analysis. C Left panels: low magnification image depicting the selective stereotaxic infusion of AAV-hSyn-DIO-hM3Dq-mCherry in the CA1. Scale bar: 200 μm. Right panels: representative images of CA1 NeuroTrace/PV/mCherry labelling showing overlapping signals (yellow) of mCherry (red) and PV + interneurons (green). Scale bars: top, 250 μm; below, 50 μm. D Percentage estimation of PV-IN infected with the hM3Dq AAV (PV+mCherry+/total PV+). In box-and-whisker plots the centre lines denote median values, edges are upper and lower quartiles, whiskers show minimum and maximum values and points are individual experiments (N = 7 for hM3Dq). E–F sIPSCs in CA1 neurons from hM3Dq PV_Ambra1+/− females injected with Veh (left) or CNO (right). Scale bar: 1 s, 20 pA. CNO increased the inhibitory tone onto CA1 neurons. E Peak amplitude (p = 0.013, Mann-Whitney test). F Instantaneous frequency. N = 8, 17 neurons, from 3 Veh- and 5 from CNO females. Data are expressed as mean ± s.e.m. *p < 0.05.

Back to article page