Fig. 5: Chemogenetic activation of inhibitory PV-IN in Ambra1+/− females normalizes hippocampal mRNA ERs. | Translational Psychiatry

Fig. 5: Chemogenetic activation of inhibitory PV-IN in Ambra1+/− females normalizes hippocampal mRNA ERs.

From: Chemogenetic rectification of the inhibitory tone onto hippocampal neurons reverts autistic-like traits and normalizes local expression of estrogen receptors in the Ambra1+/- mouse model of female autism

Fig. 5

A, B Histogram reports the relative expression (2-ΔΔCt) of estrogen receptors α (ERs α) and β (ERs β) in the hippocampus of Wt and Ambra1+/− females. Baseline levels of ER α and β are significantly decreased in Ambra1+/− females compared to Wt females. (Wt (N = 6), A (N = 11), for ERα: t(15)=2.76, p = 0.0145; for ERβ: t(15)=2.746, p = 0.015). C, D Histogram reports the relative expression (2-ΔΔCt) of estrogen receptors α (ER α) and β (ER β) in the hippocampus of PV_Wt/Veh females and PV_A females injected with Veh or CNO. ER α and β levels are decreased in PV_Ambra1+/− compared to PV_Wt females. C ER α deficit is significantly rescued in PV_A/CNO females, whereas no differences in (D) ER β level are found in PV_A/CNO females, as compared to their vehicle counterpart. [For ER α: PV_Wt/Veh (N = 6), PV_A/Veh (N = 6) and PV_A/CNO (N = 6); F(2,15)=6.96, p = 0.007; PV_Wt/Veh vs PV_A/Veh p = 0.05, PV_A/Veh vs PV_A/CNO p = 0.007. For ER β: PV_Wt/Veh (N = 7), PV_A/Veh (N = 7) and PV_A/CNO (N = 5); F(2,16)=2.761, p = 0.09; PV_Wt/Veh vs PV_A/Veh: t(12)=2.410, p = 0.032; PV_A/Veh vs PV_A/CNO: t(10)=1.04, p = 0.32)]. *p < 0.05, **p < 0.01.

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