Fig. 5: Effects of SA on depression-like phenotype, spleen weight and pro-inflammatory cytokines after LPS injection. | Translational Psychiatry

Fig. 5: Effects of SA on depression-like phenotype, spleen weight and pro-inflammatory cytokines after LPS injection.

From: A role of splenic heme biosynthesis pathway in the persistent prophylactic actions of arketamine in lipopolysaccharide-treated mice

Fig. 5

A Experimental protocol. SA (120 mg/kg/day) or saline (10 ml/kg/day) was administered i.p. to mice for consecutive 3 days before injection of LPS (1.0 mg/kg) or saline (10 ml/kg). Locomotion test and forced swimming test (FST) were performed 23 and 24 h after the injection of saline or LPS, respectively. Blood and spleens were collected after behavioral tests. B Body weight change (one-way ANOVA: F2,25 = 72.50, P < 0.0001). C Locomotion test (one-way ANOVA: F2,25 = 1.298, P = 0.291). D FST (one-way ANOVA: F2,25 = 15.38, P < 0.0001). E Spleen weight (one-way ANOVA: F2,25 = 36.37, P < 0.0001). F Plasma levels of TNF-α (one-way ANOVA: F2,25 = 6.748, P = 0.004). G Plasma levels of IL-6 (one-way ANOVA: F2,25 = 6.456, P = 0.006). H There was a positive correlation (R = 0.452, P = 0.0157) between spleen weight and plasma TNF-α. I There was a positive correlation (R = 0.576, P = 0.0013) between spleen weight and plasma IL-6. J Alas2 mRNA in the spleen (one-way ANOVA: F2,25 = 14.60, P < 0.0001). K Fech mRNA in the spleen (one-way ANOVA: F2,25 = 14.39, P < 0.0001). L Hmbs mRNA in the spleen (one-way ANOVA: F2,25 = 15.42, P < 0.0001). The data represent mean ± S.E.M. (n = 9 or 10). *P < 0.05, **P < 0.01, ***P < 0.001. N.S., not significant.

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