Table 1 Antibody panel used in flow cytometry assays to identify cereblon-binding proteins in myeloma cells
Fluorochrome | V450 | V500-C | Alexa Fluor488/FITC | PE | PerCP | APC |
|---|---|---|---|---|---|---|
Control tube (clone, company) | / | CD138 (MI15, BD) | / | / | / | CD38 (MHCD3805, Life Technologies) |
Tube 1 (clone, company) | CD45 (2D1, BD) | CD138 (MI15, BD) | IKZF1 (R32–1149, BD Pharmingen) | IKZF3 (S50–895, BD Pharmingen) | CD56 (5.1H11, BioLegend) | CD38 (MHCD3805, Life Technologies) |
Tube 2 (clone, company) | CD45 (2D1, BD) | CD138 (MI15, BD) | KPNA2 (orb188808, Biorbyt) | / | CD56 (5.1H11, BioLegend) | CD38 (MHCD3805, Life Technologies) |
