Fig. 2: Validation of 9p24.1 copy number gain and ampilfication by FISH.

Tissue microarray (TMA) slides containing patient DLBCL samples were used for the FISH studies. Fifty nuclei were analyzed for each sample. Normal indicates two target signals and two control signals, gain was defined as a target:control probe ratio of 1–2 and an average target:control probe signal < 6, and amplification was defined as a target:control probe ratio > 2 or a target probe signal ≥ 6. Representative images of no gain (a), gain (b), and amplification (c) of 9p24.1 are shown