Table 1 Target antigens, antibody clones, and dilutions for markers used.
From: Circulating Th17 T cells at treatment onset predict autoimmune toxicity of PI3Kδ inhibitors
Antigen | Antibody dilution | Clone | Manufacturer | Opal Fluorochrome |
|---|---|---|---|---|
CD3 | 1:1000 | poly | Dako | 620 |
CD8 | 1:7000 | C8/144B | Dako | 540 |
CD4 | 1:250 | 4B12 | Dako | 520 |
FOXP3 | 1:2000 | 206D | Biolegend | 570 |
Granzyme B | 1:100 | Grb-7 | Dako | 690 |
RORγT | 1:150 | 6F3.1 | BioCare Medical | 650 |
