Fig. 8 | Bone Research

Fig. 8

From: Ciliary parathyroid hormone signaling activates transforming growth factor-β to maintain intervertebral disc homeostasis during aging

Fig. 8

Shear stress enhances transport of PTH1R to primary cilia. a Immunostaining for acetylated α-tubulin or PTH1R showing that shear stress stimulated transport of PTH1R to primary cilia of both mouse and human NP cells. b Quantitative analysis of PTH1R intensity in mouse (left) and human (right) cilia of (a). c Immunostaining for acetylated α-tubulin or PTH1R showing that PTH-stimulated transport of PTH1R to primary cilia of NP cells in the presence of compression loading in an IVD ex vivo compression model of 3-month-old and 30-month-old rat. d Immunostaining of the mouse and human NP cells treated with pallidin siRNA or control siRNA for acetylated α-tubulin or PTH1R showing that PTH-stimulated transport of PTH1R to primary cilia of NP cells was inhibited. Scale bar, 10 μm. e Quantitative analysis of PTH1R intensity in mouse (left) and human (right) cilia of (d). f Western blot analysis of PTH-induced pCREB levels in NP cells treated with pallidin siRNA or control siRNA. n = 8 per group

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