Fig. 1

Different levels of resistance to anoikis among osteosarcoma cell lines. a The volumes of tumors derived from different osteosarcoma cell lines in a subcutaneous implantation tumor model. n = 5 in each group; the data are presented as the means. Sphere cultures of U2OS, MG63, HOS, and 143B cells propagated for 7 days (b) and quantification (c). n = 12 in each group; the data are presented as the means. 3D cultures of U2OS, MG63, HOS, and 143B cells with Matrigel for 2 weeks (d) and quantification (e, f). n = 5 in each group; the data are presented as the mean ± s.d. values. Representative Western blots of anoikis-related proteins in U2OS cells in monolayer or floating culture for 3 days (g) and quantification (h). n = 3 in each group; the data are presented as the mean ± s.d. values. Representative Western blots of anoikis-related proteins in 143B cells in monolayer or floating culture for 3 days (i) and quantification (j). n = 3 in each group; the data are presented as the mean ± s.d. values. The graphs show the individual data points derived from three independent measurements and the means. *P < 0.05; ****P < 0.000 1; ^###P < 0.001; ^####P < 0.000 1. Scale bars, 50 μm