Fig. 5

Mutant TP53 in transformed osteosarcoma cell lines. a TP53 mutations in HOS and 143B cells were identified using Sanger sequencing. b Representative structure of amino acid substitutions (red) in the mutant TP53 protein in HOS and 143B cells. Representative Western blots of TP53 in U2OS, MG63, HOS, and 143B cells (c) and quantification (d). n = 3 in each group; the data are presented as the mean ± s.d. values. Staining for TP53 (yellow) in U2OS and 143B cells cultured on glass surfaces (e) and quantification (f). n = 5 fields in each group; the data are presented as the mean ± s.d. values. g Expression of TP53 target genes in U2OS and 143B cells cultured on glass surfaces. n = 3 in each group. The graphs show the individual data points derived from three independent measurements and the means. ***P < 0.001; ****P < 0.000 1; ^###P < 0.001. Scale bars, 50 μm