Fig. 6

Mutant TP53 inhibits rigidity sensing in transformed osteosarcoma cells. a Expression of rigidity-sensing genes in U2OS and 143B cells. n = 3 in each group. Representative Western blots of TP53-silenced 143B cells (b) and quantification (c). n = 3 in each group; the data are presented as the mean ± s.d. values. d Expression of rigidity-sensing genes in TP53-silenced 143B cells. n = 5 in each group; the data are presented as the mean ± s.d. values. Staining for paxillin (green) in TP53-silenced 143B cells (e) and quantification (f). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Staining for actin (red) in TP53-silenced 143B cells cultured on rigid (40 kPa) or soft (4 kPa) hydrogel surfaces overnight (g) and quantification (h). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Soft agar cultures of TP53-silenced 143B cells for 2 weeks (i) and quantification (j, k). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Sphere cultures of TP53-silenced 143B cells propagated for 7 days (l) and quantification (m). n = 5 fields in each group; the data are presented as the mean ± s.d. values. The graphs show the individual data points derived from three independent measurements and the means. *P < 0.05; **P < 0.01; ***P < 0.001. Scale bars, 50 μm