Fig. 2

SEs are involved in MSC osteogenesis. a ARS and ALP staining showing that BRD4 knockdown and overexpression affect MSC osteogenesis. Quantification of ARS and ALP is shown in the scatter plots. b Western blot analysis showing that BRD4 knockdown and overexpression affect COL I expression in MSCs. c BRD4 knockdown and overexpression affect osteogenesis in vivo. HE, Masson and COL I immunohistochemistry staining of HA/TCP. Scatter plots showing Masson staining quantification. d ARS and ALP staining of MSCs treated with DMSO or 50 nmol·L⁻¹ JQ1. Quantification of ARS and ALP is shown in the scatter plots. e COL I protein abundance in osteogenic-differentiating MSCs treated with DMSO or 50 nmol·L⁻¹ JQ1. The relative intensity of COL I is shown in the scatter plot. f Effects of JQ1 on osteogenesis in vivo. HE, Masson and COL I immunohistochemistry staining of HA/TCP. Scatter plot showing Masson staining quantification. g CUT&Tag profile heatmap of BRD4 in MSCs treated with DMSO or 50 nmol·L⁻¹ JQ1. h Western blot analysis showing BRD4 expression in MSCs from the NCs (n = 21) and OP patients (n = 17). i Immunofluorescence showing BRD4 expression in the femurs of the NCs (n = 21) and OP patients (n = 17). The statistical data are represented as the means ± SEMs, n = 9 (except h, i), *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001