Fig. 4
From: Super enhancers targeting ZBTB16 in osteogenesis protect against osteoporosis
ZBTB16 plays a pivotal role in SE-mediated osteogenesis but is decreased in OP. a Heatmap of DEGs between MSCs not undergoing osteogenic induction and MSCs during osteogenic differentiation. b Volcano plot showing the DEGs of MSCs in the OB and NC groups. c GO analysis showing the osteogenic-related terms. d GSEA showing the enriched osteogenic-related terms between the NC and OB groups. e Venn diagram showing the intersection of OB-gain SEs in all datasets, significantly upregulated genes in OB MSCs, and DEGs in OP MSCs compared to those of the NC subjects. The log2fc of the 15 intersected genes are shown. f Signal traces of RNA-seq and ChIP-seq data. The shadows show the SE regions. g Scatter plot showing the expression of ZBTB16 mRNA in osteogenic differentiating MSCs. h Immunoblot analysis showing the protein abundance of ZBTB16 in osteogenic differentiating MSCs. Scatter plot showing the relative abundance of ZBTB16. i Western blot analysis showing ZBTB16 expression in MSCs from the NCs (n = 21) and OP patients (n = 17). j Immunofluorescence showing BRD4 and ZBTB16 expression in the femurs of the NCs (n = 21) and OP (n = 17) patients. k ChIP‒qPCR analysis showing BRD4 occupancy on ZBTB16 in MSCs of the NCs (n = 21) and OP (n = 17) patients. The statistical data are represented as the means ± SEMs, n = 9 (except i–k), *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001
