Fig. 6
FGF signaling modulates mechanotransduction genes Piezo2 to regulate differentiation of progenitors and WNT signaling. a–f Expression of Piezo2 in Fgfr1fl/fl and Gli1-CreER;Fgfr1fl/fl mice at PN7.5. White arrowheads point to the expression of Piezo2 in the apical papilla and follicle; white arrows point to increased Piezo2 in the apical, middle, and coronal papilla and apical follicle. White dashed lines outline HERS. The Schematic at the bottom indicates the induction protocol. g Relative expression of Piezo2 in pCMV6 and pPiezo2-treated group with qPRC in vitro. P = 0.000 3, unpaired Student’s t-test, n = 3 and each point represents one biological replicate. h–k Expression of Piezo2 and Ibsp in pCMV6- and pPiezo2-treated apical mesenchymal cells from control mice. l–q Mineralized nodules with Alizarin red staining in control, pCMV6- and pPiezo2-treated apical mesenchymal cells from control mice. r Quantification of calcium deposition in the three groups. P < 0.000 1, one-way ANOVA, n = 3 biologically independent samples. s, t Knockdown of Piezo2 in apical mesenchymal cells from Gli1-CreER;Fgfr1fl/fl mice with Piezo2 siRNA treatment. u, v Expression of Ctnnb1 after Piezo2 siRNA treatment. ***P < 0.001, ****P < 0.000 1. Scale bars, 100 μm
