Fig. 6

Conditional deletion of VHL in ECs had no appreciable effects on defect healing. Representative images of MicroCT (a1&b1) and MPLSM images (a2-9&b2-10) of the vessel networks around bone defect scanned from dura periosteum. Panel a are images obtained from control VHL^f/f mice and panel b are images from VHL^ΔEC mice. Images (a6-9&b6-10) are reconstructed at 100 μm below surface to show the vessel networks in bone tissue. Quantitative MicroCT analyses to compare bone formation (c) and defect area (d) in VHL^f/f and VHL^ΔEC mice. Quantitative MPLSM analyses to illustrate the volume fractions of the total CD31⁺ vessels (e), CD31⁺EMCN⁺ (f), CD31⁺EMCN⁻ arterial vessels (g). Col 1a (2.3) GFP⁺ cells as Cyan, SHG as white, CD31as red and EMCN as green. Isolated CD31⁺EMCN⁺ and CD31⁺EMCN⁻ vessels are shown as green and red as indicated. Scale bar = 200 μm. n = 4, *P < 0.05 by ANOVA. Histologic H&E staining of the defects (h) from VHL^f/f and VHL^ΔEC mice outlining the area of new bone at the repair site. Quantitative histomorphometric analyses (i) to show changes of total new bone formation in VHL^f/f and VHL^ΔEC mice. n = 4, P > 0.05 by Student’s t-test