Fig. 5

C-MYC promotes the expression of HDAC5. a Bioinformatics analysis of the public dataset (GEO: GSE9574) for the correlation between C-MYC and HDAC5 (n = 29, untreated ER⁺ breast cancers, P = 0.0041 and R squared = 0.2669). b Western blots of C-MYC and phosphorylated C-MYC (Ser62) in parental and TAMR MCF-7 cells. c qRT-PCR of HDAC1-11 and SIRT1-7 mRNAs in MCF-7 TAMR cells with shNC and shC-MYC. d Western blot of C-MYC in TAMR MCF-7 cells transfected with shC-MYC. e Western blot of HDAC5 in TAMR MCF-7 cells transfected with shC-MYC. f qRT-PCR of HDAC5 mRNA in TAMR MCF-7 cells with shNC and shC-MYC. g Western blot of Flag and HDAC5 in TAMR MCF-7 cells transfected Flag-C-MYC plasmid. h qRT-PCR of HDAC5 mRNA in TAMR MCF-7 cells transfected with Flag-C-MYC plasmid. i ChIP-qPCR analysis with the promoter of HDAC5 following the antibody against C-MYC in TAMR MCF-7 cells. j Dual-luciferase reporter assay for HDAC5 promoter in TAMR MCF-7 cells co-transfected C-MYC or control plasmid with Renilla Luciferase plasmids. Data are representative of means ± SEM of three independent experiments (unpaired t-test, *p < 0.05, **p < 0.01 and ***p < 0.001)