Fig. 3: Decreased expression of SLC2A3 influences the effect of vitamin C. | British Journal of Cancer

Fig. 3: Decreased expression of SLC2A3 influences the effect of vitamin C.

From: Decreased vitamin C uptake mediated by SLC2A3 promotes leukaemia progression and impedes TET2 restoration

Fig. 3

a GLUT3 expression was measured in four AML cell lines and three DLBCL cell lines using western blotting. b The GLUT3 mRNA level was measured in four AML cell lines and three DLBCL cell lines using qPCR. RNA from OCI-AML3 cells served as the control to compare the relative expression in AML cell lines, and RNA from OCI-LY1 cells serves as the control to compare the relative expression in DLBCL cell lines. c The proliferation-suppressing effect of vitamin C was determined by performing cell viability assays. Four AML cell lines and three DLBCL cell lines were treated with increasing doses of L-AA (up to 500 μM) for 48 h. d The cytoplasmic vitamin C level was measured using the ascorbic acid assay kit; the cells were treated with 250 μM L-AA for 48 h, *p < 0.05, **p < 0.01 (two-way ANNOVA).

Back to article page