Fig. 2: SCD1 expression is upregulated in gastric cancer and associated with poor prognosis. | British Journal of Cancer

Fig. 2: SCD1 expression is upregulated in gastric cancer and associated with poor prognosis.

From: Stearoyl-CoA-desaturase-1 regulates gastric cancer stem-like properties and promotes tumour metastasis via Hippo/YAP pathway

Fig. 2

a Validation of top five proteins identified by iTRAQ analysis with western blotting. b Relative expression of SCD1 in metastatic cancer sites, gastric cancer tissues and adjacent non-tumour tissues from the TCGA and PLAGH databases. c, d Overexpression of SCD1 protein predicts worse survival of GC patients both in the TCGA and PLAGH databases (statistical significance was evaluated by log-rank test, *p < 0.05, ****p < 0.0001). e SCD1 expression was analysed by qRT-PCR in gastric cancer tissue and the corresponding adjacent tissues, and the data were displayed as the △Ct value (n = 93). f Western blotting of SCD1 protein in gastric cancer cell lines and the normal gastric epithelium cell line (GES-1). g WB and qRT-PCR analysis of SCD1 expression in SCD1–KD1, SCD1–KD2 and SCD1–KD3 HSC034 cells and in control. h WB and qRT-PCR analysis of SCD1 expression in HSC034 cells treated with A939572 (0, 5 and 10 μM/ml). Error bars indicate standard deviation (SD) of the mean value. *p < 0.05 and **p < 0.01.

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