Fig. 8: The PI3K/AKT pathway is the most important pathway for PZH to affect tumor metastasis via ANRIL. | Cancer Gene Therapy

Fig. 8: The PI3K/AKT pathway is the most important pathway for PZH to affect tumor metastasis via ANRIL.

From: ANRIL promotes the regulation of colorectal cancer on lymphatic endothelial cells via VEGF-C and is the key target for Pien Tze Huang to inhibit cancer metastasis

Fig. 8

A Analyzing the intersection of two transcriptomic results. B, C p-PI3K/PI3K and p-AKT/AKT ratios in orthotopic xenograft tumors with or without PZH treatment. Data represent the mean ± SEM of experiments conducted in triplicate. ***, ****P < 0.01. D, E p-PI3K/PI3K and p-AKT/AKT ratios in HCT-8/ANRIL cells with or without 0.75 mg/mL PZH treatment, compared with HCT-8/Vector cells with or without 0.75 mg/mL PZH treatment. F The concentration of VEGF-C in supernatant of HCT-8/ANRIL with or without 0.25 mg/mL PZH treatment, compared with HCT-8/Vector cells with or without 0.25 mg/mL PZH treatment. G, H p-PI3K/PI3Kratios, p-AKT/AKT ratios, MMP-2, MMP-7 and MMP-9 expression of HLECs incubated with supernatants of HCT-8/ANRIL cells with or without 0.25 mg/mL PZH treatment, compared with HCT-8/Vector cells with or without 0.25 mg/mL PZH treatment. Data represent the mean ± SD of experiments conducted in triplicate. One-way ANOVA followed by Tukey’s test was used to evaluate the statistical significance, different letters represent significant differences between different groups (P < 0.05).

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