Fig. 6: AIF deficiency induced mitochondrial alterations and a significant drop in the cellular ATP levels in thymocytes | Cell Death & Differentiation

Fig. 6: AIF deficiency induced mitochondrial alterations and a significant drop in the cellular ATP levels in thymocytes

From: AIF loss deregulates hematopoiesis and reveals different adaptive metabolic responses in bone marrow cells and thymocytes

Fig. 6

a Representative electron micrographs of AIF+/Y and AIF−/Y thymocytes. The black arrows mark the mitochondria. Bar: 0.5 µm. b Left, OCR of AIF+/Y and AIF−/Y thymocytes under basal conditions (initial rates) and in response to sequential treatment with Oligomycin, FCCP, and Rotenone/Antimycin A. Arrows indicate the time of the addition of each reagent. Right, Basal and maximal OCR of thymocytes expressed as a histogram (n = 3 independent experiments). c ΔΨm assessment in AIF+/Y and AIF−/Y 21-day-old animals (n = 18 mice per group). d Mitochondrial ROS levels measured in thymocytes from AIF+/Y and AIF−/Y 21-day-old animals (n = 18 mice per group). e Mitochondrial mass of AIF+/Y and AIF−/Y thymocytes from 21-day-old animals (n = 18 mice per group). f Glucose uptake measured by the assimilation of the d-glucose analog 2-NBDG in AIF+/Y and AIF−/Y thymocytes from 21-day-old mice (n = 5 mice per group). g ATP levels recorded in AIF+/Y and AIF−/Y thymocytes from 21-day-old animals (n = 20 mice per group). Statistical significance was calculated by Mann–Whitney test. Bars represent mean ± SEM

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