Fig. 7

KAT2B and P300 license type I IFN-dependent expression of Zbp1, Mlkl, caspase-11, and Gsdmd upon A. baumannii infection. a The expression of Hat1, Kat2b, and P300 was analyzed in WT, Irf3^−/−Irf7^−/−, and Ifnar^−/− BMDMs infected with A. baumannii for 6 h. b–d The expression of Mlkl, caspase-11, Gsdmd, and Zbp1 was analyzed in A. baumannii infected and uninfected WT BMDMs transfected with Hat1 siRNA b, Kat2b siRNA c, and P300 siRNA d. e LDH release analysis of A. baumannii (MOI 50) infected WT BMDMs transfected with Hat1 siRNA, Kat2b siRNA, or P300 siRNA for 6 h. Data are representative of three independent experiments and are means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns not significant. Med uninfected, A. bau A. baumannii infected, siCT siRNA control, siHat1 siRNA Hat1, siKat2b siRNA Kat2b, siP300 siRNA P300