Fig. 2
From: Hif1a inactivation rescues photoreceptor degeneration induced by a chronic hypoxia-like stress
Consequences of chronically activated HIF transcription factors in rods. a Retinal morphology was tested at 11 weeks, 4 months, 6 months, and 1 year of age as indicated. Cre-negative Vhlflox/flox mice served as controls. RPE retinal pigment epithelium, POS photoreceptor outer segments, PIS photoreceptor inner segments, ONL outer nuclear layer, INL inner nuclear layer. Scale bar: 50 μm. N ≥ 3. b ONL thicknesses in indicated strains were determined at 11 weeks and 6 months of age and are presented as spidergrams. Shown are means ± SD of N ≥ 3, except for Vhlflox/flox mice at 6 months (N = 2). c Expression of Gnat1 and Gnat2 in rodΔVhl (V), rodΔVhl;Hif1a (VH1), rodΔVhl;Hif2a (VH2), and rodΔVhl;Hif1a;Hif2a (VH1H2) mice at 11 weeks and 6 months of age. Expression levels were calculated relative to their respective Cre-negative controls (set to 1; dotted line). Shown are means ± SD of N = 3-4. *P < 0.05; **P < 0.01. Individual comparisons between Cre-positive and Cre-negative mice of each genotype and time point were done using Student’s t-test
