Fig. 3
From: Hif1a inactivation rescues photoreceptor degeneration induced by a chronic hypoxia-like stress
RPE phenotype in rodΔVhl mice at 4 months of age. a Fundus imaging and OCT scans of rodΔVhl;Hif1a mice (left, served as controls) and rodΔVhl mice (right) at 4 months of age. Red lines indicate the positions of the OCT scans. White arrows point to RPE irregularities in rodΔVhl mice. b Morphology of a control and three different rodΔVhl mice at 4 months of age. Shown are the focused regions where RPE irregularities were detected. Arrows: examples of pyknotic nuclei. c Higher magnifications of the RPE of a control and a rodΔVhl mouse. Red lines indicate borders of the RPE. d RPE flatmounts of rodΔVhl;Hif1a and rodΔVhl mice as indicated. Green: F-actin stained with phalloidin. Blue: nuclei stained with DAPI. RPE retinal pigment epithelium, POS photoreceptor outer segments, PIS photoreceptor inner segments, ONL outer nuclear layer, INL inner nuclear layer, Scale bars: 100 μm (a) and 50 µm (b–d)
