Fig. 6
From: Brf1 loss and not overexpression disrupts tissues homeostasis in the intestine, liver and pancreas
Expression of human BRF1 rescues loss of Brf1 liver phenotype. a IHC for BRF1, p53 and p21 on liver samples from mice harvested at day 8 post-induction. Mouse genotypes are indicated above the panels. WT mice are a combination of AhCre Brf1+/+ mice and AhCre negative mice, Hom mice are AhCre Brf1fl/fl and Hom TG mice are AhCre Brf1fl/fl HprtLSLBRF1 mice containing an extra copy of human BRF1. b Graph depicting mouse liver weight as a fraction of total body weight of animal harvested 8dpi. Genotypes are indicated on the x-axis. WT mice are a combination of AhCre Brf1+/+ mice and AhCre negative mice (n = 11), Hom mice are AhCre Brf1fl/fl (n = 11) and Hom TG mice are AhCre Brf1fl/fl HprtLSLBRF1 mice containing an extra copy of human BRF1 (n = 6). P-values are calculated for a non-parametric Mann–Whitney test. Changes between WT and Hom/TG mice are not significant whereas changes between Brf1fl/fl and WT animals are significant (p < 0.05). c Graphs showing levels of the specified liver enzymes in the blood of mice with the indicated genotypes. WT mice are a combination of AhCre Brf1+/+ mice and AhCre negative mice (n = 8), Hom mice are AhCre Brf1fl/fl (n = 5) and Hom TG mice are AhCre Brf1fl/fl HprtLSLBRF1 mice containing an extra copy of human BRF1 (n = 5). P-values are calculated for a non-parametric Mann–Whitney test. Changes between WT and Hom/TG mice are not significant
