Fig. 5
From: ATF3 promotes erastin-induced ferroptosis by suppressing system Xc–
ATF3 represses the SLC7A11 promoter independent of p53. a p53-null PC3 cells were transfected with the SLC7A11 promoter reporter with or without ATF3/p53 for dual luciferase activity assays. b The p53-binding site (p53-BS) was deleted from the SLC7A11 promoter. The mutant promoter (mtp53BS) was transfected into PC3 cells with or without ATF3 or p53 for dual luciferase activity assays. c The sequence of the predicted p53-binding site in the human SLC7A11 promoter was compared with that in the mouse promoter and the consensus p53-binding sequence. Red color marks the four nucleotides crucial for p53 binding. The nucleotides that do not match the consensus sequence are underlined. The data are presented as mean ± SD
