Fig. 3: GABPA regulates BC cell proliferation.

Three independent experiments were performed. Bars: SD. a Cell number changes resulting from the manipulation of GABPA expression. GABPA depletion accelerates while its overexpression slows down cell proliferation rates. b Enhanced clonogenic capabilities resulting from the GABPA depletion. J82 and SW1710 cells were treated with GABPA siRNAs for 48 h and colony formation then determined. c Inhibition of clonogenic capabilities mediated by GABPA overexpression. Control and GABPA-expressed J82 and HT1197 cells were compared for their ability to form colonies. d BrdU incorporation for the cellular replication assay. Shown are representative images and percentages of BrdU⁺ cells are calculated based on three independent experiments. Scale bars: 10 μm. e Cell cycle analyses of J82 and SW1710 cells with GABPA knockdown for 48 h. Shown are representative images and percentages of cells at G0/1 and S/G2/M are calculated based on three independent experiments. f Cell cycle analyses of J82 cells expressing ectopic GABPA. Shown are representative images and percentages of cells at G0/1 and S/G2/M are calculated based on three independent experiments. C Control siRNA, G1 and G2 GABPA siRNA1 and 2, G GABPA expression vector