Fig. 4: Both Fpn^fl/fl/NEXcre and AD mice developed features of ferroptosis.

A Transmission electron microscopy pictures of perinuclear area of hippocampal neurons from Fpn^fl/fl, Fpn^fl/fl/NEXcre, WT and APPswe/PS1dE9 mice at 9 months old. WT were age-matched wild type littermates of the transgenic mice. B Mitochondrial area frequency in perinuclear compartment of these mice. Calculated from n > 100 mitochondria from n > 10 pictures of 3 mice per group. C The MDA content and D the GSH content in the hippocampus of Fpn^fl/fl, Fpn^fl/fl/NEXcre, WT and APPswe/PS1dE9 mice at 9 months old. WT were age-matched wild type littermates of the transgenic mice (n = 5). E The protein levels of Gpx-4 were detected in the hippocampus of the WT and APPswe/PS1dE9 mice and F Fpn^fl/fl, Fpn^fl/fl/NEXcre mice. β-Actin was served as a loading control. G The mRNA level of ACSF, IREB2, CS, RPL8, ATP5G3 and PTGS2 were detected in the hippocampus of the WT (n = 5) and APPswe/PS1dE9 mice (n = 5) and H Fpn^fl/fl (n = 5), Fpn^fl/fl/NEXcre mice (n = 5). β-Actin was used as an internal control, and results are shown as fold change of the control. Data are shown as the mean ± SD of at least three independent experiments. Statistical analyses were carried out using two-way ANOVA and mutiple t-tests. *p < 0.05; **p < 0.01; ***p < 0.001. Detailed Statistical analyses are included with the Supplementary Table S4.