Fig. 2: Kdm6b deficiency reduces the size of medullary compartment and the number of mTECs. | Cell Death & Differentiation

Fig. 2: Kdm6b deficiency reduces the size of medullary compartment and the number of mTECs.

From: Critical role of histone H3 lysine 27 demethylase Kdm6b in the homeostasis and function of medullary thymic epithelial cells

Fig. 2

a Representative images of hematoxylin and eosin (H&E) staining of the thymus and morphometric analysis of cortical and medullary areas represented as a ratio (20 sections per thymus of 3 pairs of Kdm6bfl/flFoxn1-Cre mice and wild-type controls). b Flow cytometric profiles of thymic epithelial cells (CD45−EpCAM+) from 4-week-old wild-type and Kdm6bfl/flFoxn1-Cre mice for the cell surface Ly51 and UEA-1 lectin (left). Frequency and absolute number of cTECs (Ly51+UEA-1−) and mTECs (Ly51−UEA-1+) are shown (right) (n = 10 per genotype). c Flow cytometric profiles of Aire-expressing mTECs in the whole mTECs (CD45−EpCAM+Ly51−UEA+) from 4-week-old wild-type and Kdm6bfl/flFoxn1-Cre mice. The ratio of Aire+ mTECs to Aire- mTECs, as well as the absolute numbers of them are shown in the graphs on the right (n = 6 per genotype). d Flow cytometric profiles of mTECs from 4-week-old mice for the expression of cell surface MHCII and CD80(left). The ratio of CD80hi mTECs to CD80low mTECs, as well as the absolute numbers of them, are shown in the graphs on the right (n = 6 per genotype). e The percentage and absolute numbers of mTECs and cTECs in wild-type and Kdm6bfl/flFoxn1-Cre mice during the first two weeks (n = 3 per genotype). f Immunofluorescent staining of thymus sections from indicated mice at the age of postnatal day 0 (PN0), 4 weeks and 8 weeks with Keratin-5(K5), UEA-1 and Keratin-8(K8). Scale bars, 500 μm (n = 4 per genotype).

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