Fig. 4: AVAN expression significantly alleviates IAV virulence in transgenic mice. | Cell Death & Differentiation

Fig. 4: AVAN expression significantly alleviates IAV virulence in transgenic mice.

From: Long noncoding RNA AVAN promotes antiviral innate immunity by interacting with TRIM25 and enhancing the transcription of FOXO3a

Fig. 4

A The genotype of C57BL/6J TG mice was identified by PCR of mouse tail DNA and RT-PCR of tissue RNA. LG: lung; TH: Thymus. B, C Four-week-old TG and WT mice were inoculated with 105.125 TCID50 BJ501 virus, respectively. Survival rates and body weight changes of wild-type mice (n = 10 for each group) were monitored for 2 weeks following BJ501 challenge (*p < 0.05; **p < 0.01). D Viral load of lung tissues (n = 3 for each group) at 0, 1, 3, 5, 7, 9, 11, 14 DPI (*p < 0.05; **p < 0.01). E HE-stained images of lung tissues at 0, 1, 3, 5, 7, 9, 11, 14 DPI (magnification = ×200). F Neutrophil recruitment in the bronchoalveolar lavage fluid (BALF) (n = 3 for each group) at 0, 1, 3, 5, 7, 9, 11, 14 DPI (**p < 0.01). G The secretion of IL-8 in the BALF (n = 3 for each group) at 0, 1, 3, 5, 7, 9, 11, 14 DPI (*p < 0.05; **p < 0.01). I Wet-to-dry ratio of lung tissues (n = 5 for each group) at 5 DPI (**p < 0.01). H IL-8, IFN-α, and IFN-β expression in the lung of TG mice measured by qRT-PCR (**p < 0.01; ***p < 0.001).

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