Fig. 8: Disruption of Sirt5 enhances p53-dependent radio-sensitivity in testes, spleens and intestines, but not in livers of mice.
From: Repression of p53 function by SIRT5-mediated desuccinylation at Lysine 120 in response to DNA damage
A, B Testicular tissue from Sirt5+/+p53+/+, Sirt5−/−p53+/+, Sirt5+/+p53−/−, and Sirt5−/−p53−/− mice untreated or after 5 Gy (Gray) IR (Ionizing radiation) treatment. TUNEL staining was used for detecting apoptotic cells. Quantitation is shown B. Data show mean ± SEM; Student’s two-tailed t-test. Scale bar = 50 µm. C, D Spleen tissue from Sirt5+/+p53+/+, Sirt5−/−p53+/+, Sirt5+/+p53−/−, and Sirt5−/−p53−/− mice untreated or after 5 Gy IR treatment. TUNEL staining was used for detecting apoptotic cells. Quantitation is shown (D). Data show mean ± SEM; Student’s two-tailed t-test. Scale bar = 150 µm. E, F Intestine tissue from Sirt5+/+p53+/+, Sirt5−/−p53+/+, Sirt5+/+p53−/−, and Sirt5−/−p53−/− mice untreated or after 5Gy IR treatment. TUNEL staining was used for detecting apoptotic cells. Quantitation is shown (F). Data show mean ± SEM; Student’s two-tailed t-test. Scale bar = 200 µm. G Liver tissue from Sirt5+/+p53+/+, Sirt5−/−p53+/+, Sirt5+/+p53−/−, and Sirt5−/−p53−/− mice untreated or after 5 Gy IR treatment. TUNEL staining was used for detecting apoptotic cells. Scale bar = 150 µm.
