Fig. 2: PRL2 deficient monocytes and macrophages have increased osteoclastogenic capacity in vitro. | Cell Death & Differentiation

Fig. 2: PRL2 deficient monocytes and macrophages have increased osteoclastogenic capacity in vitro.

From: HSC70 mediated autophagic degradation of oxidized PRL2 is responsible for osteoclastogenesis and inflammatory bone destruction

Fig. 2

A Representative TRAP staining images (left panel) of BMMs isolated from Ptp4a2fl/fl mice (referred to as WT) and Ptp4a2fl/flLysMCre+ mice (referred to as KO) stimulated with M-CSF and indicated concentrations of RANKL. Percentage (%) of TRAP-positive multinucleated cells (TRAP+ MNCs) and number (#) of TRAP+ MNCs per well (right panel). (Scale bar, 100 μm). B Transcript levels of Nfatc1, Ctsk, Mmp9, and Acp5 in WT and KO BMMs with or without RANKL for 4 days. C Representative immunoblot images (upper panel) and quantification of protein levels of NFATc1 (lower panel) in WT and KO BMMs with or without RANKL for 2 days. D Transcript levels of Nfatc1, Ctsk, Mmp9, and Acp5 in WT and Ptp4a2 knockout (referred to as KO) RAW 264.7 cells with or without RANKL for 2 days. E Representative immunoblot images (upper panel) and quantification of protein levels of NFATc1 (lower panel) in WT and KO RAW 264.7 cells with or without RANKL for 2 days. F Resorption pit assay (left panel) and quantification of bone resorption area (right panel) of WT and KO mature osteoclasts. (Scale bar, 100 μm) The data are representative of two or four independent experiments (biological replicates) and shown as the mean ± s.e.m., *P < 0.05, **P < 0.01, ***P < 0.005, unpaired two-tailed Student’s t-test.

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