Fig. 5: Immune evasion is involved in GCT formation. | Cell Death & Differentiation

Fig. 5: Immune evasion is involved in GCT formation.

From: Lineage tracing of mutant granulosa cells reveals in vivo protective mechanisms that prevent granulosa cell tumorigenesis

Fig. 5

A Heat map revealing increased expressions of Cd24a and Cd47 in GCT cells. B Heat map of regulation of macrophage polarization markers associated genes in different groups, indicating the imbalance of M1/M2 macrophages in the GCTs. C q-PCR analysis of Cd24a and Cd47 expression in indicated groups, confirming the transcriptomic result of high Cd24a and Cd47 mRNA levels in GCT cells. Data indicate means ± SD; **P < 0.01; ***P < 0.001, two-way ANOVA. D Relative Cd24a and Cd47 mRNA expression levels in the non-tumor DKO ovaries and GCTs, showing higher expression levels of both Cd24a and Cd47 in GCTs than that in the non-tumor DKO ovaries. Data indicate means ± SD, *P < 0.05; ***p < 0.001, unpaired t test. E PCR results of CD47 and CD24 transcripts in the KGN cell compared to the control, showing the expression of CD47 and CD24 in KGN cell line. Blank: negative control. F Immunochemistry staining of CD47 (arrows) and CD24 (arrowheads) in human normal ovaries and GCTs, showing more CD47 and CD24 positive cells in the GCT tissues. Scale bar: 100 μm. G Statistic results of F. Data represent mean ± SD. **P < 0.01, unpaired t test.

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