Fig. 6: HIF1A-As2 promotes cell proliferation via MYC target genes. | Cell Death & Differentiation

Fig. 6: HIF1A-As2 promotes cell proliferation via MYC target genes.

From: Long non-coding RNA HIF1A-As2 and MYC form a double-positive feedback loop to promote cell proliferation and metastasis in KRAS-driven non-small cell lung cancer

Fig. 6

A Expression of the indicated MYC targets upon MYC OE or KD in H1299 and H460, respectively. B Immunoblotting analysis of the indicated proteins upon MYC KD, HIF1A-As2 KD and DHX9 KO in H1299 and CALU1. C Correlation analysis between HIF1A-As2 and MYC targets or DHX9 and MYC targets in TCGA LUAD dataset (n = 540). D Administration of MYC reversed the regulation of the MYC targets by HIF1A-As2 in H1299 and H460. E Reporter assay showing restored MYC attenuated the increased luciferase activities of p21 reporter upon HIF1A-As2 KD or DHX9 KO. Rel Luc, relative luciferase. F Colony assay showing the MYC hampered the HIF1A-As2-regulated colony formation in H1299 cells. G DHX9 regulates cell growth via MYC and p21 in H1299 cells. Data show mean ± S.D (n = 3). **p value < 0.001, *p value < 0.05 by two-tailed Student’s t test.

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