Fig. 8: HIF1A-As2 ASO inhibits tumorigenesis in vivo. | Cell Death & Differentiation

Fig. 8: HIF1A-As2 ASO inhibits tumorigenesis in vivo.

From: Long non-coding RNA HIF1A-As2 and MYC form a double-positive feedback loop to promote cell proliferation and metastasis in KRAS-driven non-small cell lung cancer

Fig. 8

A Tumor growing curves of PDX mice by intravenous inject with Ctrl, 10058-F4, ASO#1 and ASO#1 + 10058-F4. n = 4 per group. B Ensembl BLAST tool showing the pairwise sequence alignment of the human HIF1A-As2 against the mouse genome. Black arrow shows the ___location of the most likely candidate gene on the mouse chromosome. C Representative images of high-power magnifications of lungs harvested from KRASLSLG12D mice treated with CRE +/ASO Ctrl or CRE +/ASO#1. Scale bar, 1 mm. Lung parenchyma affected by several epithelial lesions is highlighted by the dashed line. D Representative images of the whole lung sections from the two groups of mice as indicated in (C). Scale bar, 2 mm. E and F Representative images (E) and quantification (F) of IHC staining for MYC and p21 in the indicated groups from KRASLSLG12D mice treated with CRE−, CRE +/ASO Ctrl or CRE +/ASO#1. C–F Group GRE- n = 7, Group CRE +/ASO Ctrl n = 5, Group CRE +/ASO#1 n = 7. G Proposed model demonstrating the role and underlying mechanism of KRAS/HIF1A-As2/DHX9/MYC circuit in NSCLC pathogenesis and metastasis. Data show mean ± S.D. **p value < 0.001, *p value < 0.05 by two-tailed Student’s t test.

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