Fig. 4: TRAF2 promotes K63-linked polyubiquitination of p62 at K420 residue and lysosome-dependent degradation.
From: The TRAF2-p62 axis promotes proliferation and survival of liver cancer by activating mTORC1 pathway
A, B p62 half-life. HCC cells and Traf2-deletion MEF cells were treated with CHX (100 μg/ml) for indicated time periods. C TRAF2 promotes ubiquitination of p62 rely on RING ___domain. D MEF cells with CHX (100 μg/ml) were treated with MG132(20 μM), CQ (50 μM), and MG132 with CQ separately for indicated time periods. E, F TRAF2 promotes K63 polyubiquitination of p62 at K420site. G HepG2 cells were transfected with FLAG-tagged p62 plasmid or mutant forms, then treated with CHX (100 μg/ml) for indicated time periods, following IB with FLAG and ACTIN Abs.
